Specific microscopy

Microscope designed to image large samples or to retrieve additional information on the protein or DNA/RNA composition of tissues. 

Nikon A1R multiphoton :

The multiphoton is a confocal microscope using a pulsed infrared laser to gain even more depth of field in the sample than a conventional confocal. 

With the resonant scan tool of the Nikon multiphoton we can quickly scan a sample to obtain large Z stacks or fast images in in vivo experiments.

Useful for large non-transparent specimens or in vivo experiments. (Nikon A1R MP technical sheet)

 Zeiss Lightsheet Z1 :

illuminate samples with a thin sheet of light, and the emitted fluorescence is then collected at 90° by a wide-field detection system. Moving the sample through the sheet of light enables rapid acquisition of 2D optical sections, which can be combined to generate a 3D image of the sample.

Useful for large transparent specimens or cleared samples. (Zeiss Lightsheet Z1 technical sheet)Nikon TIRF HILO Fiche Descriptive  

Leica LMD7000 : 

Laser microdissection enables precise isolation and excision of microscopic homogeneous sub-samples from an original heterogeneous tissue section. The researcher can selectively define regions of interest, down to a single cell, excise them with a laser and collect them without contact (by gravity). (Leica LMD 7000 technical sheet ,  Leica LMD 7000 Instruction manual)

Olympus Intravital : 

The Olympus Intravital is an upright microscope that allows to observe samples or live animals with a protection against bleaching and laser photoxicity. (Olympus Intravital technical sheet).

Nikon TIRF HILO : 

Total internal reflection fluorescence (TIRF) microscopy represents a method of exciting and visualizing fluorophores present in the near-membrane region of live or fixed cells grown on coverslips ideal for visualization and spectroscopy of single-molecule fluorescence near a surface. Highly Inclined and Laminated Optical (HILO) Microscopy is an efficient illumination technique when applied to fluorescence imaging of thick samples owing to the confined illumination volume that allows high contrast imaging while retaining deep scanning capability in a wide-field configuration. (Nikon TIRF HILO technical sheet).