Spatial transcriptomics encompass a variety of methods aimed to quantify RNA expression directly from intact tissues. Spatial transcriptomics methods include in situ hybridization, in situ sequencing, and in situ capturing technologies, each with their own strengths and limitations.
Visium HD:
Visium HD enables full transcriptome analysis at cellular resolution. This technology uses slides containing continuous capture zones made up of millions of 2 x 2 µm barcoded squares, enabling precise localization of gene expression on fresh, fixed or FFPE tissue.(Visium HD Technical Sheet)
Xenium :
Xenium is a high-resolution spatial transcriptomics technology that simultaneously analyzes gene expression within a single tissue section. This platform offers a gene expression map at sub-cellular resolution, using either ready-to-use probes or customized probes. It allows the use of fresh FFPE or frozen tissue sections, where probes are hybridized to RNA targets, then amplified to generate unique barcodes, guaranteeing precise identification and localization of transcripts, with the possibility of analyzing the expression of 5000 genes. (Xenium Technical Sheet)
